Cover of: Coupled bioluminescent assays | Michael J. Corey

Coupled bioluminescent assays

methods, evaluations, and applications
  • 2.97 MB
  • 8951 Downloads
  • English
by
Wiley , Hoboken, N.J
Bioluminescence assay, Luciferases, Luminescent Measurements -- me
StatementMichael J. Corey.
Classifications
LC ClassificationsQP519.9.B55 C67 2009
The Physical Object
Paginationp. ;
ID Numbers
Open LibraryOL22665648M
ISBN 139780470108833
LC Control Number2008045532

This book enables readers to take full advantage of coupled bioluminescent (CB) assay techniques by demonstrating how they are used to solve real-world problems. It guides readers involved in drug Coupled bioluminescent assays book and biomedicine through a broad range of valuable CB techniques, including assays of kinases, phosphatases, and other enzymes; cytotoxicity Cited by: 4.

Coupled Bioluminescent Assays, explains the uses of CB technologies across drug discovery to analyze toxicity, drug receptors, and enzymes.

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It covers applications in environmental analysis and biodefense, including cytotoxicity, fertilizer and explosives analysis, and nerve agent and pesticide detection. Get this from a library.

Coupled bioluminescent assays: methods, evaluations, and applications. [Michael J Corey] -- This book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense.

It separates. Get this from a library. Coupled bioluminescent assays: methods, evaluations, and applications. [Michael J Corey] -- "This book enables readers to take full advantage of coupled bioluminescent (CB) assay techniques by demonstrating how they are used to solve real-world problems.

It guides readers involved in drug. Other Uses of Aequorin in Coupled Bioluminescent Assays, 12 Coupled Bioluminescent Reporter Assays Introduction to Reporter Assays, Brief History and Development of Reporter Assays, Other Non-Luminescent Reporter Proteins, Luciferases as Reporters of Promoter Activities,   This book highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense.

It separates theoretical aspects from the applied sections in a clear and readable way. Coupled Bioluminescent Assays, explains the uses of CB technologies across drug Author: Michael J. Corey. Coupled Bioluminescent Assays Methods, Evaluations, and Applications by Michael J.

Description Coupled bioluminescent assays FB2

Corey. Coupled Bioluminescent Assays highlights the applications of coupled bioluminescence assay techniques to real-world problems in drug discovery, environmental and chemical analysis, and biodefense. It separates theoretical aspects from the applied sections in a clear and readable.

bioluminescent analysis and ecological biophysics with a particular inter est in design of bioluminescent enzymatic assays for monitoring the wide range of.

amples of bioluminescent assays designed for HTS are shown in Fig. The linear range of these assays can be enormous because of the very low backgrounds in bio-luminescence, typically extending four to eight orders of magnitude across the concentration of the variable com-ponent.

Most recently, new assay formats have been de. This review addresses recent progress made in the use of bioluminescent assays for HTS, highlighting new detection capabilities brought about by. The signal-to-background ranges of the bioluminescent and radioactive assays applied from to 50, cells were similar (– and –, respectively).

Moreover, f to 50, cells, the average Coupled bioluminescent assays book rate of glucose uptake was ± fmol/min/cell for the radioactive assay and ± fmol/min/cell for the Cited by: 5.

Peptide hormones perform important biological functions by binding specific cell membrane receptors. For hormone–receptor interaction studies, receptor-binding assays are widely used.

However, conventional receptor-binding assays rely on radioactive tracers that have drawbacks. In recent studies, we established novel non-radioactive receptor-binding assays Cited by:   We recently developed novel bioluminescent binding assays for several protein/peptide hormones using the brightest bioluminescent reporter known to date, nanoluciferase (NanoLuc).

The NanoLuc reporter can be either chemically conjugated to an appropriate position, or genetically fused at one terminus, of protein/peptide by: 6.

Author by: Lisa K. Minor Languange: en Publisher by: CRC Press Format Available: PDF, ePub, Mobi Total Read: 15 Total Download: File Size: 52,5 Mb Description: The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery.

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Bioluminescent reporter gene assays have a distinct advantage over fluorescent assays, such as green fluorescent protein (GFP), in that they can deliver to 1,fold higher assay sensitivity.

This greatly increased sensitivity can substantially improve assay performance when applied to complex biological samples (2). Bioluminescent assays couple a limiting component of a luciferase-catalyzed photon-emitting reaction to a variable parameter of interest, while holding the other components constant or non-limiting.

In this way light output varies with the parameter of interest. This review describes three bioluminescent assay types that use firefly luciferase Cited by: The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery.

The Handbook of Assay Development in Drug Discovery is a reference that describes the complete armament of tools currently available for performing various assay techniques. ATP Bioluminescence Assay Kit CLS II. ATP dilutions in a volume of 50 l w ere assayed with 50 l luciferase reagent in a black mi crotiter plate on a Berthold LB 96 P luminometer.

The light signal was in tegrated for 10 s after a delay of 1 s. Figure 2:File Size: KB. A representative compound (PubChem CID: ) was resynthesized from the scaffold 1 cluster series and we confirmed an IC 50 of ∼80 nM in both bioluminescent assays (Fig.

5C). The opposite nature of the luminescent response observed for each assay (Fig. 5C) provided a good assay pair to confirm genuine Clk4 by: Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a name was first used by Raphaël Dubois who invented the words luciferin and luciferase, for the substrate and enzyme, words are derived from the Latin word lucifer – meaning lightbringer.

Organism: Photinus pyralis. This animation shows how powerful and flexible the bioluminescence reaction can be when applied to cell-based assays. Background The real-time monitoring of polynucleotide amplification is at the core of most molecular assays.

This conventionally relies on fluorescent detection of the amplicon produced, requiring complex and costly hardware, often restricting it to specialised laboratories. Principal Findings Here we report the first real-time, closed-tube luminescent reporter system for nucleic.

This review article, which originally appeared in ASSAY and Drug Development Technologies, discusses recent progress made in the use of bioluminescent assays for HTS, highlighting new detection capabilities brought about by engineering luciferase genes, enzymes, and substrates.

The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery. The Handbook of Assay Development in Drug Discovery is a reference that describes the complete arm.

Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and.

The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery.

The Handbook of Assay Development in Drug Discovery is a reference that describes the complete armament of tools currently available for performing various assay. Oracle Database 11g a Beginner's Guide by Ian Abramson and Michael Abbey and Michael J. Corey and a great selection of related books, art and collectibles available now at   The need to screen targets faster and more efficiently, coupled with advances in parallel and multiplex chemical synthesis, has contributed to the increasing use of multiwell assays for drug discovery.

The Handbook of Assay Development in Drug Discovery is a reference that describes the Price: $ Bioluminescence imaging (BLI) is based on oxidation of a substrate (a luciferin) by an enzyme (a luciferase), which usually requires energy (FMNH 2 and ATP), Mg 2 +, and oxygen to generate bioluminescent photons (Qin et al., ).There are five basic luciferin–luciferase systems (Table ).The most widely studied are those belonging to luminous beetles in the families.

Bioluminescence Assays Promega Bio-Glo™ Luciferase Assay System The Bio-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous reagent, the Bio-Glo™ Assay reagent with a new luciferase substrate, for the detection of firefly luciferase reporter gene expression in the ADCC Reporter Bioassay.Preparation of Luciferin for In Vitro Bioluminescent Assays Materials • D-Luciferin Firefly, potassium salt, g /vial (PerkinElmer Part Number #) • Sterile water • Complete media Cells should be seeded in a plate overnight or several hours prior to assay to allow the cells to attach to the Size: KB.Bioluminescent sensor allows detection of molecular processes in live cells through reversible allosteric modulation of luminescence activity during ligand binding.

Binding of ligand, in this case cAMP, results in a closed conformation of the biomolecule and concomitant increase in .